Grapical abstract high fat diet rats

All rats were executed at the 6th weekend. Address correspondence to: We therefore investigated whether HFD affects brain Hb concentration as well. Quantitative densitometry was carried out by the Gel-Pro Analyzer software right.

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Exp Clin Endocrinol Diabetes Here we demonstrate, for the first time, that Hpt protein level is significantly reduced in hippocampus of rats fed for a long term until 12 or 24 weeks a high fat diet. At the end of the 3rd wk, the BW increment in group B.

Insulin resistance and excessive accumulation of lipids is strongly associated with non-alcoholic fatty liver disease NAFLDwhich represents the hepatic manifestation of a systemic impairment of the insulin network [ 14 ]. Indeed ALT was higher 1.

These findings led us to hypothesize that Hpt decrease might enhance the oxidative stress induced by free Hb. Wistar and SD rats grapical abstract high fat diet rats assigned for 2 experimental groups for 17 weeks: Comparison of mean values in groups was conducted with single-factor analysis of variance one-way analysis of varianceand pairwise comparison was performed with the least significant difference method.

The calibration curve was obtained by assaying the immunoreactivity of 2, 1, 0. The PCR was performed under the following conditions: Primary reagents The following reagents were obtained: Therefore, the identification of molecules that can be modulated by dietary fats, might contribute to clarify the specific obesity-related effects on brain physiology and pathology.

Peroxidase-catalyzed color development from o-phenylenediamine was measured at nm. This impairment of microvascular blood flow occurs early in the pathogenesis of T2D, with evidence at the time of diagnosis [ 24 ].

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Br J Nutr Each interventional group was administered with B. Analysis of Hb and N-Tyr Concentration in Hippocampus Free Hb is highly toxic Alayash,due to the presence of heme, that disrupts the lipid bilayers, and to the well known ability of iron to cause protein oxidation in vivo and in vitro Qu et al.

Hpt expression in rat hippocampus. Further, we have recently reported that Hpt also affects ApoE-dependent 24 S -hydroxycholesterol esterification La Marca et al. Animal models have contributed greatly to the study of diabetes.

As known, Hpt binds with high affinity free Hb, thus preventing Hb-related oxidative damage and also limiting the release of heme Quaye, Lipo-LP treatment also impaired Hpt secretion, inducing a 2. Changes of fasting blood glucose and 2-h blood glucose of HFD-fed rats Monthly samples were collected from fasting rats for measurements of plasma levels of glucose and insulin.

In addition to genetic predisposition [ 2 ], physical inactivity [ 1 ], and perinatal environment [ 13 ], such diets are recognized as major causes of the obesogenic environment in humans [ 1 ].

In contrast, Hpt mRNA did not change in response to dietary intervention. Insulin resistance was evaluated using the homeostasis model assessment HOMA2. However, a direct comparison between Wistar and SD Rat as models of high-fat HF diet-induced obesity has not been adequately evaluated so far.

The other half of the tissue was rapidly washed with warm phosphate buffer pH 7. This pro-oxidative effect of Hb could be amplified by the reduction of Hpt, the major Hb-scavenger. Measuring C-peptide was preferred to measuring insulin for evaluating insulinemia, because it is more stable in blood and is not affected by haemolysis [ 31 ].

Band intensities were calculated as integrated optical density IOD.

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Histological analysis showed that the ASE-treated group showed lowered numbers of lipid droplets and smaller size of adipocytes compared to the HFD group. Each sample was experimentally analyzed in triplicate. Pharmacol Rep Am J Physiol Endocrinol Metab Each histologic section was observed for 5 fields of high power field.

Altogether our data, identifying Hpt as a molecule modulated in the brain by dietary fats, may represent one of the first steps in the comprehension of the molecular mechanisms underlying the diet-related effects in the nervous system. Model and control groups were given equivalently 0.

High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat

After this adaptation period, 48 rats were randomly assigned to six dietary treatment groups, 8 rats in each group. · Energy intake, weight gain and body fat mass of Wistar and Sprague-Dawley (SD) rats fed either with standard (St) or high-fat (HF) diet during 17 weeks Energy ingestion was higher in HF diet groups of both strains since the beginning of the study (P Location: Rockville Pike, Bethesda, MD.

Abstract The aim of this study was to investigate the anti-obesity and antihyperlipidemic efficacy and molecular mechanisms of Aster spathulifolius Maxim extract (ASE) in rats with high-fat diet (HFD)-induced obesity. Rats were separately fed a normal diet or a HFD for 8 weeks, then they were treated with ASE (,or mg/kg) for Cited by: 8.

In contrast, the rats fed with high-fat diet (HFD) develop obesity, hyperinsulinemia, and insulin resistance and not frank hyperglycemia or diabetes, thus limiting the screening of agents on controlling the blood glucose level.Cited by: Changes of (A) body weight (g) and (B) consumption of food (g/day/rat) of the HFD- fed rats.

Values are mean ±S.E.M. for rats per group. ND, normal diet; HFD, high fat diet. · Abstract. Effects of capsaicin, a pungent principle of hot red pepper, were studied in experiments using male rats fed a diet containing 30% by: Synergic effect of vitamin A and high-fat diet in adipose tissue development and nuclear receptor expression in young rats - Volume Issue 4 - Anabelle Redonnet, Carine Ferrand, Céline Bairras, Paul Higueret, Catherine Noël-Suberville, Pierrette Cassand, Claude AtgiéCited by:

Grapical abstract high fat diet rats
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